Investigating processes in the in vitro cell cultures are aimed to follow the proliferation and life of the cells in living organism.

The skin, one of the biggest organs of our organism, is formed by cells displaying specialised functions, including epidermal cells (keratinocytes), dermis cells (fibroblasts), pigment cells (melanocytes), fat cells (adipocytes). Any type of cells  can be isolated and kept alive under laboratory conditions.  Cell co-cultures are developed, e.g. epidermal cells with pigment cells, as well as epidermis and full-thickness skin models. Therefore, three-tier 3D models are used for cosmetic safety testing.

The skin, one of the biggest organs of our organism, is formed by cells displaying specialised functions, including epidermal cells (keratinocytes), dermis cells (fibroblasts), pigment cells (melanocytes), fat cells (adipocytes). Any type of cells  can be isolated and kept alive under laboratory conditions.  Cell co-cultures are developed, e.g. epidermal cells with pigment cells, as well as epidermis and full-thickness skin models. Therefore, three-tier 3D models are used for cosmetic safety testing.